Substrates
Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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Invitrogen™ C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)
Produces a green fluorescence
Invitrogen™ DQ™ Gelatin From Pig Skin, Fluorescein Conjugate - Special Packaging
Analog of natural substrate
Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
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Invitrogen™ DQ™ Red BSA - Special Packaging
Invitrogen™ DQ™ Red BSA - Special Packaging
Hydrolysis of substrate yields brightly fluorescent product
Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
| Substrate | Beta-Gal Substrate |
|---|---|
| Assay | Reporter Enzyme |
| Substrate Properties | Chemical Substrate |
| Content And Storage | Store at -20°C. |
| Format | 96-well Plate |
| Product Type | Pierce beta-Galactosidase Assay Reagent |
| For Use With (Equipment) | Microplate Reader |
| Form | Liquid |
| Label or Dye | Enzyme Labeled |
| Target Enzyme | Beta-Galactosidase |
| For Use With (Application) | Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Invitrogen™ DQ™ Collagen, type I From Bovine Skin, Fluorescein Conjugate
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity.
Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)
Measure results in microplate assays or using flow cytometry
Invitrogen™ MUP (4-Methylumbelliferyl Phosphate, Free Acid)
MUP is a fluorescent substrate for alkaline phosphatases
Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)
Continuously assay acid phosphatases at low pH
| Shipping Condition | Room Temperature |
|---|---|
| Excitation/Emission | 358⁄455 |
| Substrate | Phosphatase Substrate |
| Substrate Properties | Chemical Substrate |
| Content And Storage | Store in freezer (-5 to -30°C). |
| For Use With (Equipment) | Microplate Reader |
| Form | Powder |
| Label or Dye | DiFMU |
| Color | Blue |
| Target Enzyme | Phosphatases |
| Detection Method | Fluorescence |