Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Fisher Chemical Pepsin, Extra Pure, Powder, SLR, Fisher Chemical™

Enzyme that breaks down proteins into smaller peptides.

Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Macherey-Nagel™ Liquid Proteinase K

Liquid Proteinase K (14-22 mg/mL, > 50 U/mL)

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

MP Biomedicals™ Elastase 2X Crystallized, from Porcine Pancreas

Activity 50 u/mg protein, Porcine Pancreas, Milky Solution

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Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix

Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

Invitrogen™ TURBO DNA-free™ Kit

Ideal tool for clearing residual DNA

Thermo Scientific™ Proteinase K, recombinant, PCR grade

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.