Crosslinking, Labeling and Protein Modification
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Invitrogen™ Alexa Fluor™ 647 C2 Maleimide
Alexa Fluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | Sulfo-SMCC |
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| PEGylated | No |
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store at -20°C protected from light and moisture. |
| Cleavable | By Thiols |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 492.4 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Nonselective |
| Cell Permeability | No |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Spacer Arm Length | 18.2 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Optimize applications where product integrity and risk minimization are paramount with this high-quality formulation of carbodiimide crosslinker.
| Content And Storage | Upon receipt store desiccated at -20°C. |
|---|---|
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| Product Line | Pierce™ |
| PEGylated | No |
Invitrogen™ Alexa Fluor™ 568 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 568 is a bright orange dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 568 dye is water soluble and pH-insensitive from pH 4 to pH 10.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 568 |
| Reactive Moiety | Active Ester, Succinimidyl Ester |
| Emission | 602 nm |
| Excitation | 578 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor™ |
| Cleavable | No |
|---|---|
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 326.26 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 7.7 Å |
| PEGylated | No |
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Invitrogen™ Click-iT™ Protein Reaction Buffer Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Click two moieties together: azide or alkyne and the corresponding click detection reagent
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
| Content And Storage | Store desiccated at -20°C, protect from moisture |
|---|---|
| Product Type | NHS-Fluorescein |
| Label or Dye | FITC (Fluorescein) |
| Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
| Solubility | DMF (Dimethylformamide) |
| Emission | 518 |
| Excitation | 494 |
| Label Type | Classic Dyes |
| Chemical Reactivity | Amine |
| Product Line | Pierce™ |
Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay.
Invitrogen™ Alexa Fluor™ 405 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 405 is a blue-fluorescent dye optimal for use with the 405 nm violet laser.
| Shipping Condition | Room Temperature |
|---|---|
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 405 |
| Reactive Moiety | Active Ester, Succinimidyl Ester |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor™ |
Invitrogen™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Fluorescein-5-Isothiocyanate |
| Label or Dye | FITC (Fluorescein) |
| Reactive Moiety | Isothiocyanate |
| Label Type | Classic Dyes |
| Chemical Reactivity | Amine |