Protein Analysis Reagents
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Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
| Assay | BCA Assay |
|---|---|
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO
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Thermo Scientific Slide-A-Lyzer™ G3 Dialysis Cassettes facilitate the rapid and trouble-free dialysis of sample volumes from 0.5 to 125 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.
| MWCO | 3.5 kDa |
|---|---|
| Format | Cassette |
| Membrane | Cellulose Membrane, Dialysis |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Slide-A-Lyzer |
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
| MWCO | 7.0 kDa |
|---|---|
| Column Type | Size-exclusion, Proprietary Resin |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Zeba |
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads
Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Antibody |
| Type | Magnetic Affinity Bead |
| Target | HA |
| Content And Storage | Store at 4°C. |
| Protein Form | Recombinant |
| Form | Liquid |
| Concentration | Slurry: 10mg/mL, 1% solids |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ C18 Spin Tips & Columns
Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.
| Content And Storage | Store at room temperature. |
|---|---|
| Workflow Step | Sample Clean Up |
| Final Product Type | Peptides |
| For Use With (Equipment) | Microcentrifuge, Mass Spectrometer |
| Starting Material | Peptides,Protease-digested Protein |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.
| Content And Storage | Upon receipt store product at 4°C. Do not freeze. |
|---|---|
| Inhibitors | Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Phosphatase Inhibitor Cocktail |
| Reagent Type | Phosphatase Inhibitor |
| Product Line | Halt |
Gibco™ FreeStyle™ CHO Expression Medium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Animal origin-free, chemically defined, protein-free medium
| Cell Line | FreeStyle™ CHO-S Cells, CHO |
|---|---|
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Product Type | Expression Medium |
| Endotoxin Level | ≤0.25 EU/mL, ≤ 0.25 EU/mL |
| Classification | Chemically Defined, Protein-Free, Serum-Free |
| Culture Type | Suspension Cell Culture |
| Phenol Red Indicator | No Phenol Red |
| Product Line | Freestyle, Gibco |
| Serum Level | Serum-Free |
| Glutamine | No Glutamine |
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Thermo Scientific™ Pierce™ HeLa Protein Digest Standard
Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
| Form | Solid |
|---|---|
| Final Product Type | Peptides |
| Product Type | Protein Digest Standard |
| For Use With (Equipment) | Mass Spectrometer |
| Reagent Type | Standard |
| Starting Material | Peptides |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | Sulfo-SMCC |
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| PEGylated | No |