Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

Separating macromolecules, such as proteins from small contaminants, is often done through routine methods including gel filtration chromatography and buffer exchange. These methods are commonly used during protein purification workflows to remove small contaminants like salts, excess labels, and cross-linking reagents from samples before analysis.

The new and enhanced Thermo Scientific Zeba Spin Desalting columns and plates are specifically designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They contain a proprietary and enhanced size-exclusion chromatography resin with a more robust bead structure, making them ideal for handling centrifugal force pressure. The strengthened bead structure results in better flow characteristics and increased efficiency in removing low MW protein contaminants from samples. The Zeba resin performs exceptionally well in purifying protein samples, even when they are significantly diluted. It eliminates over 95% of contaminants without causing protein loss.

Zeba desalting columns are the ideal solution for quick and straightforward desalting and buffer exchanging of protein samples. They are offered in variousformats, such as pre-dispensed spin columns (75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL), 96-well filter plates, and chromatography cartridges. These options make it simple to handle sample volumes between 2 μL and 4 mL.

Zeba Spin Desalting 96-well Plates provide trouble-free high throughput desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. They require no resin dispensing or hydration, and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes.

Features and Benefits

• High-performing resin—exceptional protein recovery at lower protein concentrations
• Fast and Efficient—eliminate sample dilution, fraction collection, and the need for expensive chromatography system that take time to set up and can only process one sample at a time
• Easy to use—no cumbersome column preparation or equilibration
• Flexible formats—available in spin columns and 96-well spin plates for a range of sample volumes (2 μL to 4 mL)