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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent
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Quantity:
250 mL
500 mL
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250mL
500mL
Description
Includes
Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Specifications
Specifications
| Formulation | Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease |
| Quantity | 500 mL |
| Product Type | Bacterial Protein Extraction Reagent |
| Volume (Metric) | 500 mL |
| Content And Storage | Store at 4°C. |
| Product Line | B-PER™ |
Safety and Handling
Product Identifier
- BPER Complete Bacterial Protein Extraction Reagent
- Warning
- Serious eye damage/eye irritation Category 2
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- MIXTURE LIST-Contains : Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy
For Research Use Only. Not for use in diagnostic procedures.
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