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Smad3, p Ser423, p Ser425 Antibody, DyLight 350, Novus Biologicals™

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Product Code. 18214609 Supplier Novus Biologicals Supplier No. NBP177836UV
  • ONLINE EXCLUSIVE PRICE
    741.00 EUR
    700.35 EUR / 0.1mL
    Save 40.65 EUR
    5% Off
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Estimated Shipment: 03-08-2026
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Description

Rabbit Polyclonal Antibody

Smad3 (p Ser423) Polyclonal specifically detects Smad3 (p Ser423) in Human, Mouse samples. It is validated for ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Single Cell Western, Western Blot.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Smad3 (p Ser423)
Applications ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Single Cell Western, Western Blot
Classification Polyclonal
Conjugate DyLight 350
Dilution Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
Formulation 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols SMAD3
Host Species Rabbit
Immunogen Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Purification Method Affinity Purified
Quantity 0.1 mL
Research Discipline Phospho Specific, Signal Transduction, Wnt Signaling Pathway
Primary or Secondary Primary
Gene ID (Entrez) 4088
Test Specificity This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Target Species Human, Mouse
Content And Storage Store at 4C in the dark.
Isotype IgG
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