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Thermo Scientific™ Rapid ELISA Mouse mAb Isotyping Kit
Description
Thermo Scientific™ Pierce Rapid ELISA Mouse Antibody Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.
Highlights:
Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
Specific – characterize antibodies for six different subclasses and two different light-chain types
Sensitive – accurate characterization with samples containing more than 3ng/mL of test antibody
Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
Cost effective – cost per assay is less than with single-use assay strips or cassettes
No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
Complete and easy to use – includes pre-coated plates, detecting antibody, buffers, and TMB substrate and stop solutions
Includes:
Precoated 96-well Isotyping plates, 5 plates; Goat anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30mL; TMB Substrate, 55mL; Stop solution, 55mL; 30X Wash buffer, 200mL; Tris buffered saline, 2 packs
Specifications
Specifications
| Content And Storage | Sufficient For: 60 isotype determinations (8 wells per test sample) • Pre-coated 96-well Isotyping Plates, 5 plates • Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL • TMB Substrate, 55 mL • Stop Solution, 55 mL • 30X Wash Buffer, 200 mL • TBS Buffer Packs (each makes 500 mL), 2 packs Store at 4°C. |
| For Use With (Application) | ELISA (Antigen Capture), Absorbance |
| For Use With (Equipment) | Microplate Reader |
| Quantity | 60 Tests |
| Assay | Isotyping Assay |
| Detection Method | Colorimetric |
| Product Line | Pierce™ |
| Target Specificity | Target-specific |
Safety and Handling
- Rapid ELISA Mouse mAb Isotyping Kit
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 5-chloro-2-methyl-3(2H)-isothiazolone, Sodium chloride, Tris(hydroxymethyl) aminomethane, sulphuric
For Research Use Only. Not for use in diagnostic procedures.
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