missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Product Code. 13454269 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1000 Reaction Kit
200 Reaction Kit
Unit Size:
1000 reactions
200 reactions
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
13454269 200 Reaction Kit 200 reactions
13464269 1000 Reaction Kit 1000 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 13454269 Supplier Thermo Scientific™ Supplier No. 88953

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.

Additional Information and Online Ordering

The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.

Highlights:

  • Convenient – add–mix–read assay format for adherent and suspension cells

  • Colorimetric – quantitatively measures LDH release by formation of colored product

  • Robust – uses stable LDH enzyme activity as a cytotoxic marker

  • Flexible – ideal for high–throughput screening

  • Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays

Includes:

Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control

Requires:

Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette

Recommended for:

  • Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
  • Detect cytolysis in bioreactor or 3D tissue engineering applications
TRUSTED_SUSTAINABILITY

Specifications

Description Pierce LDH Cytotoxicity Assay Kit
Quantity 200 Reaction Kit
Kit Contents Substrate Mix, lyophilizate, Stop Solution, 12mL, LDH Positive Control, 6µL, Assay Buffer, 0.6mL, 10X Lysis Buffer, 2.5mL
Sufficient For 200 reactions in 96-well plates or 800 reactions in 384-well plates
Product Title
Select an issue

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.