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Thermo Scientific™ Pierce™ Protein A/G Plus UltraLink™ Resin

Extremely durable polyacrylamide support with densely immobilized, recombinant Protein A/G for exceptional IgG purification.

Brand:  Thermo Scientific™ 53135

Product Code. 11869400

  • 612.00 EUR / 2mL
Estimated Shipment: 28-10-2024
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Description

Description

Thermo Scientific™ Pierce Protein A/G Plus UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein A/G has been immobilized at high density for use in IgG purification methods.

The Protein A/G Plus UltraLink Resin is used for high-capacity binding and purification of IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A/G is a recombinant fusion protein consisting of the antibody-binding domains of Protein A and Protein G, enabling antibody purification from serum samples of a variety of species.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

  • Immobilized Protein A/G – combines the IgG binding domain of both Protein A and Protein G, enabling purification of numerous IgG subclasses and species

  • High capacity – “Plus” variety has a dense load of immobilized Protein A/G, providing a binding capacity of at least 28mg human IgG per milliliter of resin

  • UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

  • Support pH Stability: 1 to 13
  • Average Particle Size: 50 to 80 microns
  • Exclusion Limit: greater than 2,000,000 daltons
  • Average Surface Area: greater than 250m2 per gram of beads
  • Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
  • Pore Size: 500 to 1000 angstroms (50 to 100nm)
  • Maximum Linear Velocity: 3000cm per hour
  • Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Resin
UltraLink Affinity Resin
Protein A/G
Upon receipt store resin at 4°C. Product is shipped at ambient temperature.
Pierce™, UltraLink™
Bottle
IgG Antibodies
2 mL
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For Research Use Only. Not for use in diagnostic procedures.