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Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Artikelnummer. 12122240 Alle ansehen Thermo Scientific Produkte
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Quantity:
100 Reaction Kit
1000 Reaction Kit
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12122240 100 Reaction Kit Each
12132240 1000 Reaction Kit Each
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price
Regulatorischer Hinweis: Abgabe sachkundepflichtiger Stoffe und Gemische nur bei Erfüllung der Vorrausetzungen nach Chemikalienverbotsverordnung (ChemVerbotsV). Keine Abgabe an Privatpersonen.
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86.67 EUR valid until 2026-06-26
BEST PRICE on Promo! Use promo code "27969" to get your promotional price.
Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price
Regulatorischer Hinweis: Abgabe sachkundepflichtiger Stoffe und Gemische nur bei Erfüllung der Vorrausetzungen nach Chemikalienverbotsverordnung (ChemVerbotsV). Keine Abgabe an Privatpersonen.

Beschreibung

Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.

The Thermo Scientific™ Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Highlights

  • Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
  • Cost effective—highest sensitivity assays result in decreased reagent consumption
  • Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
  • Time-saving—assays using secreted luciferases require minimal sample handling
  • Automation-friendly—amenable to high throughput screens
  • Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
  • Safe—allows one to perform non-radioactive assays

Requires

Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers.

Recommended applications

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

TRUSTED_SUSTAINABILITY

Spezifikation

Type Assay Kit
Target Luciferase, Gaussia Luciferase
Sufficient For 100 Luciferase Assays
Quantity 100 Reaction Kit
Content And Storage Sufficient For: 100 luciferase assays in microplate wells
• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)
• 100X Coelenterazine, 50 μL (store at -80°C)
• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)

Kit may be stored at -80°C, or individually at indicated temperatures.
Assay Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Detection Method Bioluminescence
For Use With (Equipment) Luminometer (Microplate)
Format 384-well plate, 96-well plate
Label Type Enzyme Labeled
Substrate Properties Chemical Substrate
Technique Enhanced Chemiluminescence
Substrate Type Luciferase Substrate
Product Line Pierce
Substrate Coelenterazine
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Sicherheit und Handhabung

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Produktidentifikator
  • Pierce Gaussia Luciferase Flash Assay Kit
 Signalwort
  • Danger
 Gefahrenkategorie
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1
 Gefahrenhinweis
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.
 Sicherheitshinweis
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Ergänzende Informationen
  • MIXTURE LIST-Contain: methanol

For Research Use Only. Not for use in diagnostic procedures.

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