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OPTIZYME™ PvuI, Fisher BioReagents™

Product Code. 11904812
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Quantity:
300 U
Unit Size:
Each
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Product Code. 11904812

Brand: Fisher BioReagents BP80501

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Description

5'...C G A T^C G...3'
3'...G C^T A G C...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 50 - 100%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 90% of the DNA fragments can be ligated and more than 95% of these can be re-cut after 50-fold over-digestion with PvuI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of PvuI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI
  • Methylation Effects:
  • Dam: Completely overlaps - no effect
    • CGm6ATCG
  • (No effect on cleavage)
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - blocked
    • CGATCG
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components >50% Glycerin, 25 to 50% Water, 1 to 2.5% Sodium Chloride
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Pvu I
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 300mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 300 U
    Cut Site CGAT.CG
    Product Type PvuI
    Form Liquid
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