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OPTIZYME™ ApaI, Fisher BioReagents™
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Quantity:
5000 U
Unit Size:
Each
Description
5'...G G G C C^C...3'
3'...C^C C G G G...5'
Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 20 - 50%
- Buffer 3: 0 - 20%
- Buffer 4: 20 - 50%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1 mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Incubation at 30°C results in a 2-fold increase in activity.
- ApaI is inhibited by salt concentrations above 50 mM.
- ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain.
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: May overlap - cleavage impaired
- 5'...GGGCCm5CW GG...3'
- 5'...GGGCCm5C G...3'
- 5'...m5C GGGCCm5C G...3'
Specifications
Specifications
| Concentration | 10 U/μL |
| Components | 25 to 50% Water, >50% Glycerin |
| Incubator Temperature | 37°C |
| pH | 7.4 |
| For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with ApaI |
| Storage Buffer | 10mM Tris HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
| Content And Storage | Keep container tightly closed |
| Quantity | 5000 U |
| Cut Site | GGGCC.C |
| Product Type | Apal |
| Show More |
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