Invitrogen™ TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit, with Mammalian TC-Tag Gateway™ Expression Vectors (Green Fluorescence) (Red Fluorescence)

The expression construct is created when the Gateway™ entry clone (containing the gene of interest) is recombined with one of two destination vectors (pcDNA6.2/nTC-Tag-DEST for N-terminal tagging or pcDNA6.2/cTC-Tag-DEST for C-terminal tagging). The expression construct is then used to transform the host strain. The protein can be detected either with green-fluorescent FlAsH-EDT₂ reagent or red-fluorescent ReAsH-EDT₂ reagent. The tag is very small (6 amino acids) and the protein of interest is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Labeling Chemistry, Lumio Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays, Tetracysteine (Lumio/FlAsH/ReAsH) Assays