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Invitrogen™ MembranePro™ Functional Protein Expression Kit

Product Code. 17173029
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Quantity:
10 reactions kit
Unit Size:
10 reactions
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Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Product Code. 17173029

Brand: Invitrogen™ A11667

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This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Description

MembranePro™ Functional Protein Expression Kit - 10rxn

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

  • Proteins are displayed on human (or other mammalian) cell membranes
  • Cellular quality control and mammalian posttranslational processing helps produce functional protein
  • Proteins bud off as lipoparticles and are easily collected from the culture medium
  • Lipoparticles are enriched with expressed receptors

    MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

    1. Clone your gene of interest using a TOPO™ vector
    2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
    3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
    4. Store lipoparticles at -80 C or proceed to downstream assays

    MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Type MembranePro Functional Protein Expression Kit
    Delivery Type Virus-Like Particles (VLPs)
    Quantity 10 reactions kit
    Content And Storage The kit contains:
    •pEF6 V5-His TOPO™ TA Vector Kit for at least 10 TOPO™ cloning reactions (Store at -20°C)
    •3 x10^6 293FT Cells (Store in liquid nitrogen)
    •1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
    •1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
    •3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
    •Manuals
    Selection Agent (Eukaryotic) Blasticidin
    Cell Line 293FT
    Expression Mechanism Cell-Based Expression
    High-throughput Compatibility High-throughput Compatible
    Selection Marker Eukaryotic BsdR
    Expression System Mammalian
    Expressed Protein Type Membrane Protein
    Product Line MembranePro™
    For Use With (Application) Membrane Protein Expression
    Promoter EF-1α
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    For Research Use Only. Not for use in diagnostic procedures.

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