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  5. Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Product Code. 10669880 Shop All Thermo Scientific Products
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Product Code. 10669880 Supplier Thermo Scientific™ Supplier No. EP0421

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Description

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit.

Highlights

  • Lacks 3'→5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers

Applications

  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • Strand displacement amplification (SDA)
  • DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion

TRUSTED_SUSTAINABILITY

Specifications

Concentration 5 U/μL
Polymerase DNA Polymerase I
Compatible Buffer Restriction Enzyme, PCR, RT Buffer
Quantity 300 units
Product Type Klenow Fragment

For Research Use Only. Not for use in diagnostic procedures.

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