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Invitrogen™ SuperScript™ Indirect cDNA Labeling System
Description
Includes
The SuperScript™ Indirect cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, oligo(dT)20- VN (2.5μg/μL), random primers, control RNA ladder (0.5g/μL), 5X first-strand buffer, 0.1M DTT, 10mM dNTP mixture, RNaseOUT™ Recombinant Ribonuclease Inhibitor (40U/μL), DEPC-treated water, DMSO (Labeling Grade), 2X coupling buffer, 3M NaAc, pH 5.2, and glycogen (20mg/mL). The SuperScript™ Indirect cDNA Labeling System includes all of the components listed for the Labeling Module, plus S.N.A.P.™ column, collection tubes, loading buffer, wash buffer, amber collection tubes.
In this indirect labeling method, mRNA or total RNA is reverse transcribed using SuperScript III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA. The template RNA is then degraded by base hydrolysis, and the reaction is neutralized with acid. The amino-modified cDNA is then purified to remove unincorporated nucleotides, primers, and buffers. In the second step, the modified cDNA is coupled with the active form of a fluorescent dye. The fluorescently labeled cDNA is purified with a S.N.A.P.™ spin column to remove any unreacted dye. The resulting fluorescently labeled cDNA is ready for hybridization to microarrays.
The SuperScript Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. (The SuperScript Indirect cDNA Labeling Module includes just the core kit reagents. Both provide the flexibility to use fluorescent nucleotides of your choice.)
- SuperScript III RT for generating high cDNA yields
- A proprietary nucleotide mixture to increase signal intensity
- A convenient kit format that saves valuable time
DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis
Specifications
Specifications
| Product Type | cDNA Labeling System |
| Detection Method | Fluorescence |
| Content And Storage | The SuperScript™ Indirect cDNA Labeling Module includes SuperScript™ III Reverse Transcriptase, oligo(dT)20- VN (2.5 μg/μl), random primers, control RNA ladder (0.5 g/μl), 5X first-strand buffer, 0.1 M DTT, 10 mM dNTP mixture, RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 U/μl), DEPC-treated water, DMSO (Labeling Grade), 2X coupling buffer, 3M NaAc, pH 5.2, and glycogen (20 mg/ml). The SuperScript™ Indirect cDNA Labeling System includes all of the components listed for the Labeling Module, plus S.N.A.P.™ column, collection tubes, loading buffer, wash buffer, amber collection tubes. Store all Labeling Module components at -20°C. Store S.N.A.P.™ columns, collection tubes, loading buffer, wash buffer, and amber collection tubes at room temperature. Guaranteed stable for 6 months when properly stored. |
| Final Product Type | cDNA (Labeled) |
| Label or Dye | Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5 |
| Includes Label or Dye | No |
| Labeling Target | cDNA |
| Labeling Method | Indirect Labeling |
| Sample Type | RNA |
| Product Line | SuperScript |
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For Research Use Only. Not for use in diagnostic procedures.
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