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Descrizione
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Order Info
Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)
Specifica
Specifica
| Bed Volume | 100 μL |
| pH Range | 3 to 12 |
| Support Type | 6% Highly Cross-linked Agarose |
| Content And Storage | 4°C to 30°C |
| Quantity | 1 Pack His SpinTrap Spin Columns (50 x 100 μL) |
| Type | Spin Column |
| Sample Volume | 600 μl |
Sicurezza e movimentazione
Identificativo prodotto
- His SpinTrap, 50 columns
- Warning
- Skin sensitiser Category 1
- H317-May cause an allergic skin reaction.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501-Dispose of contents/container to .
- MIXTURE LIST-Contain: nicke, reaction massof:5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2Hisothiazol-3-one
Titolo del prodotto
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