Biological Buffers

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 3 - 10Store in Fridge at 4C

Alfa Aesar™ Tricine, 98+%

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-(Trishydroxymethylmethyl)glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO TRICINE, 98+% 25G

Invitrogen™ UltraPure™ Glycerol

Low temperature enzyme storage and concentration 3.8 LT GLYCEROL REDISTILLED

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

GE Healthcare His Buffer Kit

Purify histidine-tagged proteins under native conditions. His Buffer Kit consists of convenient, premade buffer stock solutions optimized for histidine-tagged protein purification. HIS BUFFER KIT

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Invitrogen™ Potassium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ DNase I Buffer (10X)

MAXIscript Kit component 10X DNASE 1 BUFFER 1 MLAn Ambion® component of the MAXIscript® Kits

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 1 LT TE, PH 8.0 1L STORE AT ROOM TEMPERATURE

PIPES, 98.5+%, for biochemistry, ACROS Organics™

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: 1, 4-Piperazinebis(ethanesulfonic acid) PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 2.5KG PIPES, 98.5+%, for biochemistry

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins 1 ML PROTEINASE K SOLUTION (20 MG/ML) 1ML STOREat -20 C

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 7.0 500ML STORE AT ROOM TEMPERature

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 100mL Blocker Casein in PBS

Invitrogen™ DEPC-Treated Water

1 LT DEPC-TREATED WATER 1000ML STORE AT ROOMtemperature

Alfa Aesar™ MOPS-EDTA-sodium acetate buffer (MESA)

1LT MOPS-EDTA-sodium acetate buffer (MESA) 1l

Alfa Aesar™ MES, 0.2M buffer soln., pH 5.5

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O 500ML MES, 0.2M buffer soln., pH 5.5 500ml

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 100mL SuperBlock (TBS) blocking buffer

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 1000 MLsolution, 1000 ml

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 1 LT BLOCKING BUFFER IN PBS, STARTINGBLOCK T20 1LStore in Fridge at 4C

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