Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Invitrogen™ Potassium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 5 8Store in Fridge at 4C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TS SDS 10X RUN BUF, 5L

Laemmli SDS Sample Buffer, reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, reducing (6X) 50ml

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Thermo Scientific™ Blocker™ BSA Blocking Buffers

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML

Invitrogen™ BSA, acetylated (20 mg/mL)

Stabilizes enzymatic reactions to ensure the absence of contaminutesants ACETYLATED BSA (20MG/ML)6X25MGAmbion® Acetylated BSA is used as a carrier

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Alfa Aesar™ HEPES hemisodium salt

CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 500GR HEPES hemisodium salt

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT

Thermo Scientific™ MgCl2 (Magnesium Chloride)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Lonza™ Ficoll™ DNA Loading Buffer, 6X

Ideal for improving resolution and separation of DNA fragments DNA Loading Buffer 6X FP=5 x 1 ml

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 7.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes

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