Biological Buffers
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free
Ready-to-use, certified RNase-free
GE Healthcare Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Alfa Aesar™ Laemmli SDS Sample Buffer, reducing, 6X
For protein sample preparation to be used in the Laemmli SDS-PAGE system
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Nucleaic acid purfication
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Thermo Scientific™ Blocker™ BSA (10X) in PBS
Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ BSA, acetylated (20 mg/mL)
Stabilizes enzymatic reactions to ensure the absence of contaminutesants
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents
Commonly used buffering agent
Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase
Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Remains active under various conditions
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free
Ready-to-use, certified RNase-free
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution.
Alfa Aesar™ HEPES hemisodium salt
CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Lonza™ Ficoll™ DNA Loading Buffer, 6X
Ideal for improving resolution and separation of DNA fragments
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Invitrogen™ Digitonin (5%)
A detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System
