Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

GE Healthcare PhastGel™ Buffer Strips

Pre-prepared 3% agarose blocks containing buffer, for use in conjunction with PhastGel gradient and homogeneous gel media to generate a multiphasic buffer system during electrophoresis X10 PHASTGEL NATIVE BUFFER STRIPS

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

Invitrogen™ UltraPure BSA (50mg/mL)

Excellent PCR stabilizer BSA, ULTRA PURE(50MG/ML)5X50MGAmbion® Ultrapure BSA is a 'non-acetylated' BSA

HEPES Sodium Salt, CellPURE™, Fisher BioReagents

100GR HEPES sodium salt biotechnology performancecertified, cell culture tested, 99.5 (titration)

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

Chemical Name or Material: HEPES CAS: 7365-45-9 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Powder/Solid Assay: ≥99% Assay Percent Range: ≥99 % Molecular Formula: C8H18N2O4S Formula Weight: 238.3 Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid 100GR HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 0.5 M EDTA PH 8.0 100MLAmbion® Molecular biology grade, 0.5 mM EDTA, pH

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE GC BUFFER 6 MLML

Invitrogen™ Novex™ NativePAGE™ 5% G-250 Sample Additive

Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels NATIVEPAGE 5% G250 ADDITIVENativePAGE 5% G-250 Sample Additive is added to

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 6.7 TO 7.725mL Store in Fridge at 4C

Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)

Use with Novex Tricine Gels TRICINE SDS RUN BUF10X 500MLNovex Tricine SDS Running Buffer (10X)

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins 1 ML PROTEINASE K SOLUTION (20 MG/ML) 1ML STOREat -20 C

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 8.0 100MLAmbion® Molecular biology grade, 1M Tris, pH 8.0

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Novex™ Bolt™ Transfer Buffer (20X)

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting 125ML BOLT TRANSFER BUFFER (20X)

Invitrogen™ Novex™ Bolt™ Antioxidant

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML BOLT ANTIOXIDANT

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TRISGLYSDS RUN BUF(10X)500ML

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

PIPES, 98.5+%, for biochemistry, ACROS Organics™

Chemical Name or Material: PIPES CAS: 5625-37-6 Purity Grade: For biochemistry Assay: ≥98.50% Assay Percent Range: 98.5+% Molecular Formula: C8H18N2O6S2 Formula Weight: 302.35 Synonym: 1, 4-Piperazinebis(ethanesulfonic acid) 100GR PIPES, 98.5+%, for biochemistry

Invitrogen™ Novex™ NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

Designed for separation of small- to medium-size proteins on NuPAGE Novex Bis-Tris gels MES SDS BUFFER KIT NUPAGE(R) FOR BIS-TRIS GELS 00

Invitrogen™ Ammonium Acetate (5M)

Ready-to-use, certified RNase-free 5M AMMONIUM ACETATE 500 MLAmbion® Molecular biology grade, 5 M Ammonium

Invitrogen™ IPTG (Dioxane-Free)

Lac operon inducer 5 GR IPTG (DIOXANE-FREE) 5G STORE AT -20 C

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