Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 3 - 10Store in Fridge at 4C

Normal Goat Serum Control, Invitrogen™

Goat Control 2 ML NORMAL GOAT SERUM

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

HEPES sodium salt, >98%, MP Biomedicals™

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 100GR HEPES

Lonza™ Ficoll™ DNA Loading Buffer, 6X

Ideal for improving resolution and separation of DNA fragments DNA Loading Buffer 6X FP=5 x 1 ml

Thermo Scientific™ Tricine, ≥98.5% (Dry Basis), Ultrapure, Thermo Scientific™

CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-Tris(Hydroxymethyl)methylglycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO 1KG Tricine, 98.5+% (dry basis), Ultrapure, Thermo Scientific

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

Thermo Scientific™ Pierce™ Clear Milk Blocking Buffer

Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications. PIERCE CLEAR MILK BLOCKING BUFFER (10X), 100 ML

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Thermo Scientific™ FastDigest Buffer (10X)

FastDigest Buffer (10X) X10 BUFF FASTDIGEST 1ML

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. BLOCKER CASEIN IN PBS 1L4C Ship at Ambient temperature

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Commonly used buffering agent 500ML HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Invitrogen™ DNase I Buffer (10X)

MAXIscript Kit component 10X DNASE 1 BUFFER 1 MLAn Ambion® component of the MAXIscript® Kits

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 8.0 100MLAmbion® Molecular biology grade, 1M Tris, pH 8.0

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 500GR HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Proteinase K

Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C

Invitrogen™ UltraPure BSA (50mg/mL)

Excellent PCR stabilizer BSA, ULTRA PURE(50MG/ML)5X50MGAmbion® Ultrapure BSA is a 'non-acetylated' BSA

Laemmli SDS Sample Buffer, non-reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 25ML Laemmli SDS sample buffer, non-reducing (6X) 25ml

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots. 20 ML RESTORE FLUORESCENT WESTERN BLOT STRIPPINGbuffer 20mL

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