Biological Buffers
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
Nuclease-free water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved.
Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free
Ready-to-use, certified RNase-free
Cytiva Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Thermo Scientific™ Laemmli SDS sample buffer, reducing (6X)
Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system.
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer
Thermo Scientific™ Blocker™ BSA (10X) in PBS
Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods.
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ BSA, acetylated (20 mg/mL)
Stabilizes enzymatic reactions to ensure the absence of contaminutesants
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Remains active under various conditions
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution.
Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
Thermo Scientific™ HEPES hemisodium salt
CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Lonza™ Ficoll™ DNA Loading Buffer, 6X
Ideal for improving resolution and separation of DNA fragments
Invitrogen™ PureLink™ RNase A (20 mg/mL)
A bovine pancreatic ribonuclease that cleaves single-stranded RNA
Invitrogen™ NaCl (5 M), RNase-free
Ready-to-use, certified RNase-free
Invitrogen™ Novex™ IEF Cathode Buffer pH 3-7 (10X)
Optimized pre-mixed IEF buffers reduce variability and ensure consistent results
