Biological Buffers

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 2.5 - 5Store in Fridge at 4C

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Potassium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

GE Healthcare His Buffer Kit

Purify histidine-tagged proteins under native conditions. His Buffer Kit consists of convenient, premade buffer stock solutions optimized for histidine-tagged protein purification. HIS BUFFER KIT

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100GR HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ UltraPure™ Glycerol

Low temperature enzyme storage and concentration 3.8 LT GLYCEROL REDISTILLED

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE HF BUFFER 6 ML

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 100mL Blocker Casein in PBS

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ DNase I Buffer (10X)

MAXIscript Kit component 10X DNASE 1 BUFFER 1 MLAn Ambion® component of the MAXIscript® Kits

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT

PIPES, 98.5+%, for biochemistry, ACROS Organics™

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: 1, 4-Piperazinebis(ethanesulfonic acid) PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O 2.5KG PIPES, 98.5+%, for biochemistry

Invitrogen™ Novex™ Zymogram Renaturing Buffer (10X)

Used with Novex™ Zymogram Gels ZYMOGRAM RENAT BUF(10X)500ML

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots. 20 ML RESTORE FLUORESCENT WESTERN BLOT STRIPPINGbuffer 20mL

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Commonly used buffering agent 500ML HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 7.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Alfa Aesar™ TES, 0.2M buffer soln., pH 7.5

CAS: 70331-82-7 Molecular Formula: C6H14NNaO6S Molecular Weight (g/mol): 251.229 MDL Number: MFCD00065482 InChI Key: GUXMFAHOYNRHBI-UHFFFAOYSA-M PubChem CID: 23702152 IUPAC Name: sodium;2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonate SMILES: C(CS(=O)(=O)[O-])NC(CO)(CO)CO.[Na+] 250ML TES, 0.2M buffer soln., pH 7.5 250ml

Invitrogen™ TAE (25X)

Tested for RNase and DNase contaminutesation X10 25X TAE STORE AT ROOM TEMPERATURE

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 1 M TRIS, PH 8.0 100MLAmbion® Molecular biology grade, 1M Tris, pH 8.0

Alfa Aesar™ ACES, 0.5M buffer soln., pH 7.0

CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N 100ML ACES, 0.5M buffer soln., pH 7.0 100ml

Bicine, 99+%, for biochemistry, ACROS Organics™

CAS: 150-25-4 Molecular Formula: C6H13NO4 Molecular Weight (g/mol): 163.173 MDL Number: MFCD00004295 InChI Key: FSVCELGFZIQNCK-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)glycine, Diethylolglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: C(CO)N(CCO)CC(=O)O 100GR Bicine, 99+%, for biochemistry

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 500 ML WESTERN BLOT STRIPPING REAGENT, RESTORE500mL

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