Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25 ML CARRIER AMPHOLYTE, PHARMALYTE 6.7 TO 7.725mL Store in Fridge at 4C

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free 1 M MAGNESIUM CHLORIDE 100MLMgCl2solution, 100 ml

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 100mL SuperBlock (TBS) blocking buffer

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

HEPES Sodium Salt, CellPURE™, Fisher BioReagents

100GR HEPES sodium salt biotechnology performancecertified, cell culture tested, 99.5 (titration)

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility. 1 LT BLOCKING BUFFER IN PBS, STARTINGBLOCK T20 1LStore in Fridge at 4C

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 200 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 200reactions Store at -20C

Invitrogen™ dNTPs for cDNA Probe Synthesis (10 mM)

Ideal for reverse transcription DNTPS FOR CDNA SYNTHESISAmbion® dNTPs for cDNA Probe Synthesis (10 mM)

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

Chemical Name or Material: HEPES CAS: 7365-45-9 Purity Grade: Molecular Biology Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Powder/Solid Assay: ≥99% Assay Percent Range: ≥99 % Molecular Formula: C8H18N2O4S Formula Weight: 238.3 Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid 500GR HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ KCl (2M)

Ready-to-use, certified RNase-free 100 ML 2 M KCL 100ML STORE AT ROOM TEMPERATURE

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting NP TRANSFER BUF (20X)

Invitrogen™ Linear Acrylamide (5 mg/mL)

Useful for separating reaction products from unincorporated nucleotides and oligonucleotide primers LINEAR ACRYLAMIDE 5 X 1 ML

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TRISGLYSDS RUN BUF(10X)500ML

Invitrogen™ RNAsecure™ RNase Inactivation Reagent

No post-treatment autoclaving required 10 ML RNA REAGENT 10ML STORE AT -20 C

Invitrogen™ NaCl (5M)

Ready-to-use, certified RNase-free 5M NACL 500 MLAmbion® Molecular biology grade, 5 M NaCl

Invitrogen™ Novex™ NativePAGE™ 5% G-250 Sample Additive

Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels NATIVEPAGE 5% G250 ADDITIVENativePAGE 5% G-250 Sample Additive is added to

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Invitrogen™ Novex™ IEF Anode Buffer (50X)

An optimized pre-mixed IEF anode buffer IEF ANODE BUFFER (50X) 100MLNovex® IEF Anode Buffer (50X) is optimized

Invitrogen™ UltraPure BSA (50mg/mL)

Excellent PCR stabilizer BSA, ULTRA PURE(50MG/ML)5X50MGAmbion® Ultrapure BSA is a 'non-acetylated' BSA

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

GE Healthcare PhastGel™ Buffer Strips

Pre-prepared 3% agarose blocks containing buffer, for use in conjunction with PhastGel gradient and homogeneous gel media to generate a multiphasic buffer system during electrophoresis X2 Buffer strip holder GE Healthcare Phastgel

Invitrogen™ Platinum™ Pfx DNA Polymerase

Built-in hot start 500 RXN PFX DNA POLYMERASE PLATINUM(R) 500 REACTIOns Store at -20°C

BICINE, 99%, Alfa Aesar™

Chemical Name or Material: BICINE Assay Percent Range: 99% Molecular Formula: C6H13NO4 Formula Weight: 163.17 BICINE, 99% 50G

Tricine-SDS Sample Buffer, 2X, reducing

25ML Tricine-SDS Sample Buffer (2X), reducing 25m

Alfa Aesar™ TES, 0.2M buffer soln., pH 7.5

Chemical Name or Material: TES buffer soln. CAS: 70331-82-7 MDL Number: MFCD00065482 100ML TES, 0.2M buffer soln., pH 7.5 100ml

  spinner