Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Invitrogen™ Potassium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature

GE Healthcare Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 5 8Store in Fridge at 4C

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels NOVEX TRIS-GLYCINE SDS RUNNING BUFFER (10X DENATURing) . store at room temperature

Laemmli SDS Sample Buffer, reducing, 6X

For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, reducing (6X) 50ml

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents

Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology

Thermo Scientific™ Blocker™ BSA Blocking Buffers

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ BSA, acetylated (20 mg/mL)

Stabilizes enzymatic reactions to ensure the absence of contaminutesants ACETYLATED BSA (20MG/ML)6X25MGAmbion® Acetylated BSA is used as a carrier

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT

Invitrogen™ Proteinase K

Remains active under various conditions PROTEINASE K 100MG

Alfa Aesar™ HEPES hemisodium salt

CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 500GR HEPES hemisodium salt

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Thermo Scientific™ MgCl2 (Magnesium Chloride)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Lonza™ Ficoll™ DNA Loading Buffer, 6X

Ideal for improving resolution and separation of DNA fragments DNA Loading Buffer 6X FP=5 x 1 ml

Invitrogen™ Novex™ IEF Cathode Buffer pH 3-7 (10X)

Optimized pre-mixed IEF buffers reduce variability and ensure consistent results IEF CATHODE PH3-7 (10X)125MLNovex® IEF Cathode Buffer pH 3-7 (10X) is

Invitrogen™ Tris (1M), pH 7.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 7.0 500ML STORE AT ROOM TEMPERature

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 30 ML WESTERN STRIPPING REAGENT, RESTORE 30ML

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