Substrates
Invitrogen™ C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)
Produces a green fluorescence
Invitrogen™ DQ™ Red BSA - Special Packaging
Hydrolysis of substrate yields brightly fluorescent product
Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
Invitrogen™ DQ™ Green BSA - Special Packaging
Hydrolysis of substrate yields brightly fluorescent product
Novex™ AP Chromogenic Substrate (BCIP/ NBT)
Novex™ AP Chromogenic Substrate (BCIP/ NBT)
Invitrogen™ Fluorescein Diphosphate, Tetraammonium Salt (FDP)
Colorless and non-fluorescent
Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)
Cleavage produces blue-fluorescence
Invitrogen™ NBT/BCIP Reagent Kit
For chromogenic detection of phosphatase.
Thermo Scientific Chemicals Caspase-7 Substrate I, fluorogenic
For cellular and molecular biology applications
Thermo Scientific™ GFP-4E-BP1
4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
Thermo Scientific™ CDK7/9tide Substrate
A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).