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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (67)
Real Time PCR Reagents and Kits (26)

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1 – 15 of 32 results
1
Promotions Available

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

EDGE
2
Promotions Available

Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

EDGE
Shipping Condition Dry Ice
Content And Storage 2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
For Use With (Application) Animal Health,Pathogen Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Hot Start Built-In Hot Start
Product Line Ambion, Path-ID
3
Promotions Available

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type RNA
Reaction Speed Standard
Product Line AgPath-ID, Ambion
4

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
5

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe
6

Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
7

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
8

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
9
Promotions Available

Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

EDGE
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method Primer-probe
11

Applied Biosystems™ Path-ID™ qPCR Master Mix with Manual

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

Content And Storage 2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Animal Health,Pathogen Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Hot Start Built-In Hot Start
Product Line Ambion, Path-ID
12

Thermo Scientific™ Luminaris Probe qPCR Master Mixes

Ready-to-use qPCR master mixes with premixed UDG optimized for probe detection using standard cycling protocol. Master mixes available with or without blue color.

Shelf Life 2 Years
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,siRNA Validation,Pathogen Detection
13

Thermo Scientific™ Luminaris Probe Low ROX qPCR Master Mixes

Ready-to-use qPCR master mixes with premixed UDG optimized for probe detection using standard cycling protocol. Master mixes available with or without blue color.

Shelf Life 2 Years
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,siRNA Validation,Pathogen Detection
14
Promotions Available

Applied Biosystems™ VetMAX™-Plus One-Step RT-PCR Kit

For use with VetMAX™ Reagents and VetMAX™ Controls.

15
Promotions Available

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Description Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase ArrayScript™
Concentration 25X
PCR Method 1-step RT-qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type One-Step RT-PCR Reagent
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 System
Reaction Speed Standard
Product Line AgPath-ID, Ambion