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Invitrogen™ Ambion™ MessageSensor™ RT Kit
For the reverse transcription of RNA
Brand: Invitrogen™ AM1745
Additional Details : Weight : 0.06000kg
Includes: 10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, hGAPDH Control Primers, HeLa S3 Control RNA, and nuclease-free water
Description
The Ambion kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions.
- Linear quantitation of mRNA targets over 106-fold range of input RNA
- Quantitate transcripts from as little as 500fg total RNA—1/20th of a single cell
- Fast, flexible RT step complete in 15 min.; use reaction temperatures up to 50°C
- Compatible with TaqMan™, SYBR™ Green, and gel-based detection methods
- Optimal reaction temperature: 42° to 50°C
- Final product size: 7kb or less
MessageSensor is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity. The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than two hours.
PCR & Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Dry ice
Specifications
| 10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, hGAPDH Control Primers, HeLa S3 Control RNA, and nuclease-free water | |
| 50 reactions | |
| RNA |
| Separate Components | |
| cDNA Synthesis, RT-PCR |
For Research Use Only. Not for use in diagnostic procedures.