Modifying Enzymes
Invitrogen™ T4 DNA Ligase Buffer
T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
R&D Systems™ TACS Nuclease and Buffer
Highly purified recombinant endonuclease specific for all forms of DNA
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Thermo Scientific™ S1 Nuclease (100 U/μL)
Degrade single-stranded DNA and RNA with this endonuclease.
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Invitrogen™ SUMO Protease
A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.