Cell Based Assays
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)
Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters
Invitrogen™ CellMask™ Plasma Membrane Stains
Invitrogen CellMask™ plasma membrane stains enable fast and uniform labeling of the plasma membrane in almost all cell types. These stains work rapidly and can be detected using standard microscope filter sets with any imaging instrument.
Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)
General oxidative stress indicator
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
Invitrogen™ SYTO™ Green Fluorescent Nucleic Acid Stains
SYTO Green Fluorescent Nucleic Acid Stains exhibit bright, green fluorescence upon binding to nucleic acids. These dyes are cell-permeant and can be used to stain RNA and DNA in live cells, dead cells, and bacteria.
Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
Invitrogen™ MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection
Novel fluorogenic probe for measuring oxidative stress in live cells
Molecular Probes™ LIVE/DEAD™ BacLight™ Bacterial Viability Kits
LIVE/DEAD™ BacLight™ Bacterial Viability Kits are easy-to-use fluorescent staining kits commonly used for assessing the viability of bacterial cells. These kits provide a two-color fluorescence assay of bacterial viability using green and red fluorescent dyes for use with imaging, flow, and assays.
Invitrogen™ DAPI and Hoechst Nucleic Acid Stains
DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Invitrogen™ Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential.
Invitrogen™ Amplex™ Red and Amplex™ UltraRed Reagents
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Invitrogen™ MitoTracker™ Dyes for Mitochondria Labeling
These mitochondria dyes are specially packaged in vials of 50 μg each to create freshly reconstituted dyes without the impact of freeze-thaw cycles to aid in a simplified sample prep for cell analysis workflow.