PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	Medium
Target Specificity	cDNA or gDNA only
Product Line	PowerUP, SYBR

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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

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Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Pricing and Availability
Specifications

Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

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Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	>99%
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM

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Thermo Scientific™ Phusion™ Plus DNA Polymerase

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Pricing and Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Separate components
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

Pricing and Availability
Specifications

No. of Reactions	100 Reactions
Format	Kit
Label or Dye	FAM
Quantity	100 reactions
Host Cell	E.coli
PCR Method	qPCR
Product Line	resDNASEQ
Detection Method	Primer-probe

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Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

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Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Pricing and Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Standard PCR

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Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Pricing and Availability
Specifications

Polymerase	Platinum Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Starting Material	DNA
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'

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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid H Minus Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid H Minus
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA

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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems

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Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR

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Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
PCR Method	qPCR,Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	Taq DNA Polymerase
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

PCR Supplies

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