Thermo Scientific™ XLD Agar / Brilliance™ Salmonella Bi-plate

Read more results from a single patient sample and produce more diagnostic details of pathogenic organisms while reducing storage capacity issues in the incubator with a bi-plate that includes two widely used microbiological media.

XLD Agar is a selective media for the isolation and differentiation of Salmonella, relying on xylose fermentation, lysine decarboxylation and production of hydrogen sulfide for the primary differentiation of Shigella and Salmonella spp. from non-pathogenic bacteria. The sensitivity and selectivity of XLD Agar exceeds that of traditional plating media, e.g., Eosin Methylene Blue and Bismuth Sulphite agars, which tend to suppress the growth of shigellae. The recovery of salmonellae and shigellae is not obscured by profuse growth of other species; therefore, XLD Agar is ideal for the screening of samples containing mixed flora and suspected of harbouring enteric pathogens, e.g., medical specimens or food products. XLD Agar is recommended in the ISO 6579 testing of Salmonella and is tested according to ISO 11133-2.

Brilliance Salmonella Agar provides a highly selective and easy-to-read medium for the presumptive identification of Salmonella from food and environmental samples. The Inhibigen contained in this medium specifically targets Escherichia coli, with additional compounds added to suppress the growth of other non-target organisms.

Differentiation of Salmonella from other organisms is achieved through the inclusion of two chromogens that target specific enzymes: caprylate esterase and ß-glucosidase. The action of the enzymes on the chromogens result in a build-up of color within the colony, with the color produced depends on which enzymes the organism possesses. The action of caprylate esterase, present in all salmonellae, results in a purple colony.

Some other Enterobacteriaceae species also produce caprylate esterase, but these are either inhibited or differentiated from Salmonella by the ß-glucosidase substrate. This results in blue colonies which are easy to distinguish from the purple Salmonella colonies.

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