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Thermo Scientific™ Varioskan™ LUX multimode microplate reader, Absorbance, Fluorescence intensity, Luminescence and AlphaScreen

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Product Code. 15350777 Supplier Thermo Scientific™ Supplier No. VLBLA0D2

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Description

Analyze a variety of samples with five measurement technologies, including absorbance (UV-Vis), fluorescence intensity, Luminescence, AlphaScreen / AlphaLISA and TRF.

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers' best interest.

The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.

The Varioskan LUX is a modular, upgradable instrument that provides excellent usability across a variety of applications.

  • Up to five measurement technologies: Absorbance (UV-Vis), Fluorescence intensity (incl. FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA and Time-resolved fluorescence (incl. TR-FRET/hTRF)
  • Filter and monochromator optics
  • Spectral scanning for assay optimization
  • Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
  • Integrated gas control module for setting CO2 and O2 concentrations for cell based assays
  • Simultaneous dispensing and measurement for signal monitoring at the start of a reaction
  • Automatic dynamic range selection, based on signal intensity of each well
  • Smart safety controls designed to identify and alert the user of potential problems before they happen
  • Automated instrument calibration and self-diagnostics for reliable performance
  • Certified HTRF compatible reader by CisBio Bioassays
  • Validated DLReady by Promega Corporation

Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.

  • License free—Each user can install the software on his/her computer
  • Intuitive and flexible interface for multiple users and assays
  • Innovative Virtual Pipetting Tool makes it easy to define samples to plate layout
  • Measurement data is continuously saved to the database, so there's no data lost due to unexpected interruptions such as power outages or accidental aborting
  • Simplified data processing and export
    • Export data in a single mouse click, or have it emailed automatically at the end of the run
    • Several file formats for exporting results: *.xlsx, *.pdf, *.xml, and *.txt
  • Shows real-time spectra and kinetic curves during the measurement
  • Built-in calculations for data analysis (e.g. blank subtraction, average/SD/CV%, standard curve, dose response, quality control, classification, kinetic and spectral analysis, custom formula, etc.)
  • Comprehensive result reports with traceability to runtime actions and settings
  • Two editions available: Research Edition for life science researchers, and Drug Discovery Edition which offers features needed for the FDA 21 CFR Part 11 compliance.

The Varioskan LUX is modular and upgradeable, so you can build the system that best suits your needs. For more information, contact a sales representative.

TRUSTED_SUSTAINABILITY

Specifications

Compatibility Robot
Detector Type Photomultiplier Tube (PMT)
Incubator Temperature 45°C
Interface PC Software (Thermo Scientific SkanIt Software)
Light Source Xenon Flash Lamp, LED
Measurement Speed 15 s for 96-well Plate, 45 s for 384-well Plate, 135 s for 1536-Well Plate (Minimum Times)
Measurement Technology Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen
Plate Type 6 to 1536-well Plates (Absorbance 6 to 384-well Plates)
Product Line Varioskan
Reading Options Top and Bottom
Read-out Range 0 to 6 Abs
Shaking Orbital
Software Type SkanIt Software
Spectral Scanning Speed <2.2 s/Well 400 to 500 nm, 1 Flash, 2 nm Step
Wavelength Selection Absorbance & Spectral Scanning: Double Monochromator, Fluorescence Intensity: Double Excitation and Emission Monochromator, AlphaScreen: Filter
Accuracy 0.003 Abs or ±2%, at 200 to 399 nm (0 to 2 Abs)
0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
Depth (English) 23 in.
Depth (Metric) 58 cm
Dispense Volume 1 mL syringe: 2 to 5,000 μL
5 mL syringe: 5 to 25,000 μL
Dynamic Range Fluorescence intensity: top reading > 6 decades, bottom reading > 5.5 decades Luminescence: > 7 decades
Height (English) 20 in.
Height (Metric) 51 cm
Linearity 96-well plate: ±2% (0 to 4 Abs at 450 nm)
384-well plate: ±2% (0 to 3 Abs at 450 nm)
Measurement Type Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
No. of Dispensers 2
Precision SD < 0.001 Abs or CV < 0.5%, at 450 nm (0 to 3 Abs)
Sensitivity Fluorescence intensity (top reading): < 0.4 fmol fluorescein/well (black 384-well plate)
Fluorescence intensity (bottom reading): < 4 fmol fluorescein/well, (clear bottom black 384 square well plate)
Luminescence: < 7 amol ATP/well (white 384-well plate)
AlphaScreen: < 100 amol phosphotyrosine/well (384-well plate)
Software Language Versions English, German, French, Spanish, Portuguese, Italian, Japansese, Chinese and Russian
Wavelength Range Absorbance: 200 to 1000 nm
Fluorescence intensity: Excitation: 200 to 1000 nm, Emission: 270 to 840 nm
Luminescence: 360 to 670 nm
AlphaScreen: Excitation 680 nm, Emission 400 to 660 nm
Weight (English) 130 lb.
Weight (Metric) 59 kg
Width (English) 21 in.
Width (Metric) 53 cm
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Frequently Asked Questions (FAQs)

Which is the best DNA or RNA quantification instrument?

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.
What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.
Is a PC included with purchase of the Varioskan LUX Multimode Microplate Reader?

No, a PC is not included with purchase of the Varioskan LUX Multimode Microplate Reader.
For the Varioskan LUX Multimode Microplate Reader, do you have plate reader software that meets CFR 21 Part 11 regulations? Does your software have tracking function/electronic signature? Do you have a clinical version of the software?

Yes, these functions are available. However, you will need to purchase SkanIt Drug Discovery Edition Software separately (Cat. No. 5187149).
Is software included with purchase of the Varioskan LUX Multimode Microplate Reader?

Yes, SkanIt Software Research Edition is included with purchase of the reader.

Can the µDrop Plate be used with the Varioskan LUX Multimode Microplate Reader?

Yes, the µDrop Plate can be used. Prewritten protocols are available in the SkanIt Software Cloud library.
Does the Varioskan LUX Multimode Microplate Reader have quadruple monochromators?

Yes, it uses 2 excitation monochromators and 2 emission monochromators.
What is the useful lifespan of the Xenon Flash lamp in the Varioskan LUX Multimode Microplate Reader?

Typically, the lifespan is 10E9 flashes. This is equivalent to reading about 1 million 96-well microplates using 100ms measurement time per well.
With the Varioskan LUX Multimode Microplate Reader, when do I need to use pathlength correction?

Pathlength correction is typically used when measuring DNA, RNA, or protein concentrations in the UV range.
How many dispensers (injectors) can be ordered with the Varioskan LUX Multimode Microplate Reader? Can they be added after purchase?

Up to 2 dispensers (injectors) can be ordered with the Varioskan LUX Multimode Microplate Reader.

They can be added to the reader after purchase, but they must be installed by a Thermo Fisher Scientific Field Service Engineer.
Which plates can be used with the Varioskan LUX Multimode Microplate Reader?

In photometry mode, 6-well to 384-well plates can be used.
In all other read modes, 6-well to 1,536-well plates can be used.

Dispensing can be carried out with 6-well to 384-well plates.
What plate colors can be used for luminescence with the Varioskan LUX Multimode Microplate Reader?

White plates are typically used for luminescence.
With the Varioskan LUX Multimode Microplate Reader, what plate colors can be used for fluorescence intensity?

Black plates generally provide the best results; they also have the lowest background fluorescence.

Transparent or white plates can be used, but the sensitivity is often lower.
Can the Varioskan LUX Multimode Microplate Reader be integrated with robotics?

Yes, it can. To do so, you must purchase the robotic tray separately (Cat. No. N03079).

The SkanIt Automation Interface manual is available from your local support team.
Can I add gas control after purchase of the Varioskan LUX Multimode Microplate Reader?

No, the gas control module must be included with the instrument at the time of purchase. Please contact your Thermo Fisher Scientific sales representative for further information.
Can I add luminescence, TRF, AlphaScreen and dispensers (injectors) after purchase of the Varioskan LUX Multimode Microplate Reader?

Yes, all these options can be added after purchase. However, they require upgrades performed by Thermo Fisher Scientific Field Service Engineers in your lab.
What is the maximum plate height that can be used with the Varioskan LUX Multimode Microplate Reader?

The top of the plate, including any lid in use, cannot exceed the top rim of the plate tray.
The actual maximum height depends on which plate adapter is installed in the plate tray.

Adapters for plates with lids can be ordered separately.
Which plate adapters ship with the Varioskan LUX Multimode Microplate Reader?

The Varioskan LUX Multimode Microplate Reader ships with adapters for use with 6-well to 48-well, 96-well and 384 well plates without lids.

Adapters for use of plates with lids as well as 1,536-well adapter for plate without lid and 96-well adaptor for PCR plate without lid can be ordered.

https://www.thermofisher.com/order/catalog/product/N02693?SID=srch-hj-N02693
I get the following error message from the Varioskan LUX Multimode Microplate Reader with SkanIt Software: "Tip priming vessel not inserted"/"Dispenser tip priming was attempted when there were no waste wells inserted into the plate tray." How do I make this go away?

You are seeing this error because a waste strip was not detected in the plate tray.

The Varioskan LUX Multimode Microplate Reader ships with a strip plate. Remove one column of strip wells from the plate and insert into the slot on the left side of the plate tray to prevent the error message from appearing.

For details see Figure 2-15 on page 23 of the Varioskan LUX Multimode Microplate Reader technical manual.
Do I need to use filters in the Varioskan LUX Multimode Microplate Reader?

Absorbance and fluorescence intensity measurements do not use filters. For luminescence applications which require filters, users should purchase the filters separately and install them in the luminescence module.

Excitation filters for time-resolved fluorescence (TRF) and AlphaScreen are included when these options are ordered; however, emission filters must be purchased separately, as they can vary based on the user's application.

What is the wavelength range for the Varioskan LUX Multimode Microplate Reader?

In photometry mode, the wavelength range is 200 to 1000 nm (in 1 nm increments).
In fluorometry mode, the wavelength range using top reading is 200 to 1000 nm for excitation and 270 to 840 nm for emission (in 1 nm increments).

Ranges for other options can be found on our website at: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html
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