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  6. MP Biomedicals™ Trypsin, Porcine Pancreas

MP Biomedicals™ Trypsin, Porcine Pancreas

Product Code. 11402932 Shop All MP Biomedicals Products
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Quantity:
25 g
100 g
Unit Size:
100g
25g
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11402932 100 g 100g
11492922 25 g 25g
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Product Code. 11402932 Supplier MP Biomedicals™ Supplier No. 0215357180

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Description

Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

  • CAS Number: 9002-07-7
  • Molecular Weight: Approximately 23,000 or 22000 (for human)
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
  • Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
    One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
    One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
  • Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Release adherent cells from tissue culture plates for passaging
Sample Type Porcine
Content And Storage 4°C
Form Powder
Quantity 100 g
Product Type Trypsin

Safety and Handling

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Product Identifier
  • Trypsin 1:250, Virus Free
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P101-If medical advice is needed, have product container or label at hand.
  • P102-Keep out of reach of children.
  • P103-Read label before use.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: trypsin
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