TNF alpha Monoclonal Antibody (MP6-XT3), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Description: The MP6-XT3 monoclonal antibody reacts with mouse Tumor Necrosis Factor-alpha (TNF-alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4^+ T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form. Applications Reported: The MP6-XT3 antibody has been reported for use in cytokine neutralization. Applications Tested: The Functional Grade Purified MP6-XT3 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of TNF alpha bioactivity. The MP6-XT3 antibody at 0.60 μg/mL has been found to neutralize by 50% the biological effects of 1.0 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.