missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. 10218854

Cytiva Thrombin Protease

Product Code. 10218854 Shop All Cytiva Products
Change view
Click to view available options
Quantity:
500U
Unit Size:
500 units
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10218854 500U 500 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10218854 Supplier Cytiva Supplier No. 27084601

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

  • Supplied as lyophilized powder
  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

TRUSTED_SUSTAINABILITY

Specifications

Buffer Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3
Product Type Thrombin Protease Fusion Protein
Quantity 500U
For Use With (Application) Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours

Safety and Handling

compliance-icons compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : thrombin
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.