missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Brand:  Thermo Scientific™ K1691

860.62 EUR valid until 2024-03-30
Use promo code "21741" to get your promotional price.



Additional Details : Weight : 0.01000kg

Product Code. 15255146

  • 954.00 EUR / 500 reactions
In Stock
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Specifications

• RevertAid Reverse Transcriptase
• Ribolock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Random Hexamer Primer
• Nuclease-free water

Store at –20°C.


60 min.
42°C
Yes
Real Time PCR (qPCR)
500 Reactions
Reverse Transcription
RNA
Kit
Reverse Transcription
RevertAid
Dry Ice
First-Strand cDNA
Separate components
Up to 13 kb
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.