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  7. Applied Biosystems™ TaqMan™ Pri-miRNA Assay

Applied Biosystems™ TaqMan™ Pri-miRNA Assay
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Product Code. 10321815 Shop All Applied Biosystems Products
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L (2900 reactions), made to order
M (750 reactions), made to order
S (360 reactions), made to order
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10321815 S (360 reactions), made to order Each
11660129 M (750 reactions), made to order Each
11670129 L (2900 reactions), made to order Each
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Product Code. 10321815 Supplier Applied Biosystems™ Supplier No. 4427012

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Description

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway.

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.

• Highly specific—quantitiate miRNA transcription from a single genomic locus
• Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
• A new perspective—measure miRNA expression at the gene level

TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)

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Specifications

Concentration 20X
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
Product Line TaqMan
Product Type Pri-miRNA Assay
Quantity S (360 reactions), made to order
Shipping Condition Room Temperature
Sufficient For 360 Reactions
Fidelity (vs. Taq) 60 X
No. of Reactions 360 reactions
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For Research Use Only. Not for use in diagnostic procedures.

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