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Description
A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.
Order Info
Shipping Conditions: Dry Ice
Specifications
Specifications
| Concentration | 10X |
| Content And Storage | Contains 10X RT buffer, dNTP mix w/dTTp (100 mM total), RNase inhibitor (20 U/μL), and MultiScribe™ RT enzyme (50 U/μL) for 1000 reactions. Store at -15 to -25°C. |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR Method | 2-step RT-qPCR |
| Reaction Speed | Fast |
| Optimal Reaction Temperature | 42°C |
| Product Line | TaqMan |
| Product Type | MicroRNA Reverse Transcription Kit |
| Quantity | 1,000 reactions |
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For Research Use Only. Not for use in diagnostic procedures.
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