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  6. IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Column

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Column

Product Code. 16624734 Shop All IBA Lifesciences Products
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16624734 5 mL 1 set
16604734 5 x 0.2 mL 1 set
16614734 1 mL 1 set
16634734 10 mL 1 set
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Product Code. 16624734 Supplier IBA Lifesciences Supplier No. 25033001

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Description

Gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

  • Strep-Tactin™XT 4Flow™ high capacity columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin™XT and are applicable for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins via gravity flow.
  • Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins.
  • The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets.
  • In addition, Strep-Tactin™XT 4Flow™ high capacity allows the application of denaturing agents such as urea.
  • In comparison to Strep-Tactin™XT 4Flow™, Strep-Tactin™XT 4Flow™ high capacity has a higher density of immobilized Strep-Tactin™XT, which leads to a 2-fold higher yield of purified protein.
  • To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

Matrix 4% agarose, crosslinked
Particle Size 50 to 150 μm
Quantity 5 mL
Target Twin-Strep-Tag™ and Strep-tag™II
Format Pre-packed Gravity Column

For Research Use Only.

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