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Thermo Scientific™ SMART Digest™ Trypsin Kits
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Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.
Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.
SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.
Easy and simple- Significantly increased speed of sample processing
- Simple process
- Easily automate entire protocol for high-throughput processing
- Increased reproducibility over existing protocols
- Higher sensitivity
- Higher data confidence
Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.
Cleaner SamplesReduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.
Couple with SOLAμ SPE Plates for DesaltingWhen used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.
Spécification
Spécification
| Type | Trypsin Kit |
| Description | SMART Digest Trypsin Kit, with Collection plate |
| Format | Resin in Pre-filled PCR Tubes |
| Enzyme | Trypsin |
| Cleavage | C-terminal side of lysine and arginine amino acids |
| Capture | None |
| Product Line | SMART Digest |
| For Use With (Application) | Peptide Mapping |
| Includes | Resin, Collection Plate |
Sécurité et traitement
- Trypsin Kits
- Warning
- Serious eye damage/eye irritation Category 2
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- MIXTURE LIST-Contains : sodium azide, calcium chloride
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