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Cytiva Sepharose™ Fast Flow Cation Exchange Media

Artikelnummer. 10714592 Alle ansehen Cytiva Produkte
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Binding Capacity:
50 mg Ribonuclease A/mL Medium
70 mg Ribonuclease A/mL Medium
Product Type:
CM Sepharose Fast Flow Media
SP Sepharose Fast Flow Media
Packaging Quantity:
25 mL
300 mL
500 mL
Packungsgröße:
25mL
300mL
500mL
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Artikelnummer. Binding Capacity Product Type Packaging Quantity unitSize
10714592 50 mg Ribonuclease A/mL Medium CM Sepharose Fast Flow Media 500 mL 500mL
10576350 70 mg Ribonuclease A/mL Medium SP Sepharose Fast Flow Media 25 mL 25mL
10724592 70 mg Ribonuclease A/mL Medium SP Sepharose Fast Flow Media 300 mL 300mL
10059859 50 mg Ribonuclease A/mL Medium CM Sepharose Fast Flow Media 25 mL 25mL
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Artikelnummer. 10714592 Lieferant Cytiva Lieferanten-Nr. 17071901

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SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

TRUSTED_SUSTAINABILITY

Spezifikation

Quantity 500 mL
Stability Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
For Use With (Application) For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
Storage Requirements 4°C to 30°C
Anion Exchange Type Weak CatIon Exchanger
Anion Exchange Capacity 0.09 to 0.13 mM Hl+/mL
Binding Capacity 50 mg Ribonuclease A/mL Medium
Product Type CM Sepharose Fast Flow Media
Flow Rate 300 to 600 cm/Hoμr
Particle Size 45 to 165 μm
Max. Pressure 100 kPa
Matrix 6% Cross-Linked Agarose
pH Range 4 to 13
Storage Buffer 20% Ethanol
Ligand Type Carboxymethyl
Packaging Quantity 500 mL
Stationary Phase Ion Exchange Phase
Format Bulk Media
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Produktidentifikator
  • CM Sepharose Fast Flow, 500 ml
Signalwort
  • Warning
Gefahrenkategorie
  • Flammable liquid Category 3
Gefahrenhinweis
  • H226-Flammable liquid and vapour.
Sicherheitshinweis
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Ergänzende Informationen
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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