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Novus Biologicals™ Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
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Lymphotoxin-alpha/TNF-beta protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/mL, corresponding to a specific activity of > 2.0 x 10^8 IU/mg in the presence of actinomycin D.
Specifications
Specifications
| For Use With (Application) | Western Blot, Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20. |
| Gene ID (Entrez) | 4049 |
| Molecular Weight (g/mol) | MolecularWeight-theroretical: 18.7 kDa |
| Quantity | 1000 μg |
| Storage Requirements | Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.) |
| Endotoxin Concentration | Less than 0.1 EU/μg of Lymphotoxin-alpha/TNF-beta as determined by LAL method. |
| Gene Alias | LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1 |
| Gene Symbol | LTA |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.) |
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