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Proteinase K Lyophilized Powder

Product Code. 15825378 Shop All Thermo Scientific Products
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Quantity:
100 mg
Unit Size:
100mg
This item is not returnable. View return policy

Product Code. 15825378

Brand: Thermo Scientific J76230.MC

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Description

28.9 KDa

  • Used in the isolation of native, high molecular weight DNA and RNA.
  • Used as a general protease to inactivate DNases, RNases and to degrade proteins.
  • Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization.
  • Produces characteristic protein fragments used in enzyme/protein structure and function studies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

  • Lyophilized powder
  • Source: Tritirachium album
  • Concentration: 20 - 50units/mg
  • Molecular weight: 28.9kDa
  • Optimum pH: 4.0 - 12.5
  • Optimum temperature: 65°C
  • Activators: Stimulated by 0.2 - 1% SDS or 1 - 4M urea
  • Inactivated by PMSF(1); Not inactivated by EGTA, thiol reagents or trypsin/chymotrypsin inhibitors

Applications:

  • Used in isolation of native, high molecular weight DNA and RNA
  • Used as general protease to inactivate DNases, RNases and to degrade proteins
  • Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization
  • Produces characteristic protein fragments used in enzyme/protein structure and function studies
TRUSTED_SUSTAINABILITY

Specifications

Concentration 20 to 50 U/mg
Host Species T. album
Content And Storage Dry at 4°C
Quantity 100 mg
Product Type Proteinase K Powder
Molecular Weight (g/mol) 28.9 kDa
Form Lyophilized

Frequently Asked Questions (FAQs)

How should I store Proteinase K Lyophilized Powder (Cat. No. J76230.MC)?

Proteinase K Lyophilized Powder should be shipped on cold pack and stored dry at 4 degrees C upon receipt.
What is the unit definition for Proteinase K Lyophilized Powder (Cat. No. J76230.MC)?

One unit is the amount of enzyme that liberates Folin reagent-positive amino acids and peptides corresponding to 1 µmol tyrosinein 1 min at 37 degrees C using hemoglobin as substrate.
What is the working concentration of Proteinase K Lyophilized Powder (Cat. No. J76230.MC)?

The working concentration of Proteinase K Lyophilized Powder is 0.05-1 mg/ml.

RUO – Research Use Only

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