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PRKCH (PKC eta) Recombinant Human Protein

Product Code. 11579246
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Quantity:
20 μg
Unit Size:
20µg
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Product Code. Quantity unitSize
11579246 20 μg 20µg
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Product Code. 11579246 Supplier Invitrogen Supplier No. P2634

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Description

PRKCH (PKC eta)

The PKC family of serine⁄threonine kinases, including PRKCH (PKC eta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_006246
Concentration 0.33mg/mL
For Use With (Application) Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay
Gene ID (Entrez) 23683, 5583
Molecular Weight (g/mol) 82.2kDa
Name PRKCH (PKC eta) Recombinant Human Protein
Quantity 20 μg
Storage Requirements –80°C
Regulatory Status For research use only. Not for use in diagnostic procedures
Packaging 1 Each
Gene Alias PRKCH (PKC eta)
Common Name PRKCH (PKC eta) Recombinant Human Protein
Assays PRKCH (PKC eta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase Family PKC
Product Type PRKCH (PKC eta) Recombinant Human Protein
Specific Reactivity 610nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2μg/mL
Species Human
Protein Tag His Tag
Expression System Baculovirus
Protein Family Kinase and inhibitors
Protein Subtype Serine/threonine kinase
Purity or Quality Grade 92% by SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.

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