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Invitrogen™ pLenti6.3/V5-DEST™ Gateway™ Vector Kit

Product Code. 10647015
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Product Code. 10647015 Supplier Invitrogen™ Supplier No. V53306

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Description

pLenti6.3/V5-DEST™ Gateway™ Vector Kit

Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (catalog K5330-00).

The pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
  • Stable expression
  • Long-term experiments
  • Accurate titer of functional virus
  • Flexible and versatile Gateway™ recombination cloning technology

    Key Features
  • HiPerform™ WPRE and cPPT elements
  • CMV promoter
  • V5 epitope tag at C terminus
  • Blasticidin selection

    Kit includes
  • A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ
  • Stbl3™ competent cells

    For research use only. Not intended for any therapeutic or diagnostic use.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Constitutive or Inducible System Constitutive
    Delivery Type Lentiviral
    Promoter CMV
    Product Type Kit, Vector
    Selection Agent (Eukaryotic) Blasticidin
    Content And Storage Vector Kit (Box 1)
    pLenti6.3⁄V5-DEST™ (150 ng⁄μL): 6 μg, store at -20°C
    pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄μL): 10 μg, store at -20°C

    One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
    Stbl3™ Cells: 21 x 50 μL, store at -80°C
    pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C
    S.O.C. Medium: 6 mL, store at -80°C
    Cloning Method Gateway
    Quantity 1 kit
    Vector pDEST, pLenti
    Product Line Gateway, HiPerform, ViraPower
    For Use With (Application) Viral Expression
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    For Research Use Only. Not for use in diagnostic procedures.

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