missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces
BIOPHARMA

Product Code. 10143632
Change view
Click to view available options
Color:
Black
Clear
White
Product Type:
Breakable Module F8 (x12) in Frame
Lock-Well Module C8 (x12) in Frame
Microplate
Solid Module F8 (x12) in Frame
Surface Treatment:
Glutathione
Streptavidin
Total Volume (Metric) Well:
120 μL
350 μL
400 μL
Unit Size:
Pack of 15
Pack of 5
10 product options available for selection
Product selection table with 10 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Color Product Type Surface Treatment Total Volume (Metric) Well unitSize
10143632 Clear Microplate Streptavidin 400 μL Pack of 15
10387522 White Microplate Streptavidin 400 μL Pack of 15
10541493 Black Microplate Streptavidin 400 μL Pack of 15
10133682 Clear Microplate Streptavidin 120 μL Pack of 15
10551493 White Microplate Streptavidin 120 μL Pack of 15
10788944 Clear Solid Module F8 (x12) in Frame Streptavidin 400 μL Pack of 15
10636083 Clear Lock-Well Module C8 (x12) in Frame Streptavidin 350 μL Pack of 15
10709144 Clear Microplate Glutathione 400 μL Pack of 15
10723686 Clear Microplate Streptavidin 400 μL Pack of 5
10604617 Clear Breakable Module F8 (x12) in Frame Streptavidin 400 μL Pack of 5
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 10 options available.
10 options
Codice prodotto. 10143632 Fornitore Thermo Scientific™ N. del fornitore 436014
Solo null disponibili
Aggiungi al carrello
Edge
Aggiungi al carrello

Help reduce variability in molecular orientation with these plates and modules with affinity binding surfaces.

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind)

  • Highly specific binding
  • Reduced variability in molecular orientation
  • Immobilizer surfaces improve signal to noise ratios
  • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules
TRUSTED_SUSTAINABILITY

Specifica

Format Standard
Lid Without Lid
Detection Method Colorimetric
Array 8 x 12
Packaging 15 Cases
Binding Type Affinity
Color Clear
Product Type Microplate
Material Polystyrene
No. of Wells 96
Volume (Metric) Working 100 μL
Sterility Non-sterile
Surface Treatment Streptavidin
Total Volume (Metric) Well 400 μL
Well Shape Round, Flat Bottom (F-well)
No. per Case 15
No. per Pack 1
Plate Blocking Unspecified
Quantity 15/Cs.
Target Molecule Biotinylated
Well Design F384
Vedi altri risultati Mostra meno risultati
Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

Why am I getting a high background signal when using Nunc Immobilizer Streptavidin Plates/Strips?

High background signal could be due to inadequate washing. Make sure to pre-wash the Nunc Immobilizer Streptavidin Plates/Strips and increase the number of washing steps and the time between washes. Another possible cause is too high a concentration of the detection components.

How long will my Nunc Immobilizer Streptavidin Plates/Strips remain stable?

Our Nunc Immobilizer Streptavidin Plates/Strips are extremely stable over time, if stored at room temperature (20 degrees C to 25 degrees C). If kept in the unopened foil packaging, this product should have an 18 month shelf life after the date of manufacturing.

How long should I incubate the biotinylated and target molecule with the Nunc Immobilizer Streptavidin surface?

Coupling of the target molecule to the streptavidin proceeds very rapidly. Results show that incubation with gentle agitation for 2 hr at room temperature is typically sufficient to saturate all available active site.

What is the binding capacity for free biotin?

The total binding capacity for free biotin is:
- 20 pmol/well in a 96 well (coating volume 100 µL)
- 10 pmol/well in a 384 well (coating volume 50 µL)

Why should I pre-wash my Nunc Immobilizer Streptavidin?

We highly recommend pre-washing your Nunc Immobilizer Streptavidin. This will help ensure improved readouts and a very low coefficient of variation (CV% ≤ 5) from plate to plate and from well to well.

When using Nunc Immobilizer Streptavidin Plates/Strips for detection of biotinylated biomolecules, should I optimize the concentration of my target molecule?

We recommend that you optimize the amount of target molecule.
Typically, we suggest the following:
- Biotinylated oligonucleotides: 0.5 µM -0.01 µM diluted in 5 x SSCT buffer pH=7.0
- Biotinylated peptides: 1 µg/mL - 1 ng/mL diluted in a PBST buffer pH=7.2
- Biotinylated proteins: 5 µg/mL - 0.05 µg/mL diluted in a PBST buffer pH=7.2
More information regarding Nunc Immobilizer Streptavidin Plates/Strips can be found on the Thermo Scientific Nunc Immobilizer Streptavidin Instruction Protocol (link below):
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/D20693.pdf

Which types of Nunc Immobilizer Streptavidin Plates/Strips should I use for various detection methods?

We offer many types of Nunc Immobilizer Streptavidin Plates/Stripes for various applications.
We recommend:
- clear polystyrene plates and strips for colorimetric assays
- white polystyrene plates for bio- and chemiluminescence assays
- black polystyrene plates for fluorescence assays.

What types of target molecules are compatible with Nunc Immobilizer Streptavidin Plates/Strips?

When working with the Nunc Immobilizer Streptavidin instant coupling of biotinylated bio-molecules will occur. The Nunc Immobilizer Streptavidin is specifically designed and optimized for detecting biotinylated nucleic acids, peptides, and proteins.
More information regarding Nunc Immobilizer Streptavidin Plates/Strips can be found on the Thermo Scientific Nunc Immobilizer Streptavidin Instruction Protocol (link below):
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/D20693.pdf


Titolo del prodotto
Selezionare un problema

Facendo clic su Invia, l'utente riconosce che potrebbe essere contattato da Fisher Scientific in merito al feedback fornito in questo modulo. Non condivideremo le vostre informazioni per altri scopi. Tutte le informazioni di contatto fornite saranno conservate in conformità con la nostra Politica sulla privacy. Informativa sulla privacy.