missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein Labeling Reagents
  6. 10361594

Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer

Product Code. 10361594 Shop All Thermo Scientific Products
Click to view available options
Quantity:
25 mg
Unit Size:
25mg
This item is not returnable. View return policy

Product Code. 10361594

Brand: Thermo Scientific™ 46406

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.

Thermo Scientific™ TRITC and Rhodamine Labeling Reagents and Kits are high-performance derivatives of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Rhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery
TRUSTED_SUSTAINABILITY

Specifications

Description Pierce NHS-Rhodamine
Quantity 25 mg
Product Type NHS-Rhodamine
Solubility DMF (Dimethylformamide)
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Label or Dye Rhodamine Isomers, TMR (Tetramethylrhodamine), TRITC Isomers
Label Type Classic Dyes
Product Line Pierce
Emission 575
Excitation 552
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.