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Thermo Scientific™ HABA (4'-hydroxyazobenzene-2-carboxylic acid)

A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.

Brand:  Thermo Scientific™ 28010

111.33 EUR valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : CAS Number : 1634-82-8 Weight : 0.80000kg


Product Code. 11801445

  • 167.00 EUR / 10g
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Description

Description

Thermo Scientific™ Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.

Highlights:

  • HABA-avidin complex can be used over a wide range of pH and salt concentrations
  • Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
  • Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use Pierce's online HABA calculator
  • Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)
Specifications

Specifications

HABA
Upon receipt store product at room temperature. Product shipped at ambient temperature.
Biotin
10 g
Pierce™
Antibodies, Proteins
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Safety and Handling

Safety and Handling

Product Identifier
  • HABA (4'-hydroxyazobenzene-2-carboxylic acid)

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : 2-(p-hydroxyphenylazo)benzoic acid

SDS
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Documents

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For Research Use Only. Not for use in diagnostic procedures.