Learn More
Description
Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.
Highlights
- Robust and reliable exponential amplification method
- No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
- No need to destroy the starting template in a separate step
- Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
- Amplification of large plasmids up to 10 kb
- Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
- T4 DNA Ligase included in the kit; no purification steps before or after ligation
- Compatible with all strains of competent E. coli cells
Specifications
Specifications
| Form | Solution |
| Format | Kit |
| GC-Rich PCR Performance | High |
| Polymerase | Phusion Hot Start II High Fidelity DNA Polymerase |
| Reaction Speed | Fast |
| Mutagenesis Capability | Single-Site Mutagenesis |
| Product Line | Phusion |
| Product Type | Site-Directed Mutagenesis Kit |
| Quantity | 20 reactions |
| Fidelity (vs. Taq) | 52X |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.