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  5. Invitrogen™ PGFM Enzyme Competitive ELISA Kit

Invitrogen™ PGFM Enzyme Competitive ELISA Kit

Product Code. 17825017
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Quantity:
10 x 96 Tests
96 Tests
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Pack of 10
Pack of 96
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17825017 10 x 96 Tests Pack of 10
15815863 96 Tests Pack of 96
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Product Code. 17825017 Supplier Invitrogen™ Supplier No. EIAPGFMX10

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Description

ELISA

The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.

Principle of the method

The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

In many species, uterine and placental Prostaglandin F2alpha (PGFM) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F2alpha is metabolized to PGFM during the first passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2alpha and has been applied as a useful analytical marker of PGF2alpha. PGFM is identical across all species and so this kit should measure PGFM from sources other than those tested.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 50 to 3200 pg/mL
Assay Sensitivity 20.8 pg/mL
Description PGFM Competitive ELISA Kit
Assay Multiplex Assay on the Luminex Platform
Conjugate HRP
Detection Method Fluorescence
Product Type ELISA
Sample Type Fecal Extract, Plasma, Serum, Supernatant, Urine
Sufficient For 10 x 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 9.7%
Intraassay CV 9.2
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Reproductive Biology
Sample Volume Fecal Extract 50 μL, Plasma 6.25 μL, Serum 6.25 μL, Supernatant 50 μL, Urine 6.25 μL
Storage Requirements -20°C
Target PGFM
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 30 mins
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