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  5. Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Product Code. 12788622
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250 Reactions
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250 reactions
50 reactions
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Product Code. No. of Reactions unitSize
12788622 250 Reactions 250 reactions
11932512 10 Reactions 10 reactions
12798622 50 Reactions 50 reactions
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Product Code. 12788622 Supplier Macherey-Nagel™ Supplier No. 740971.250

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This item is not returnable. View return policy

Description

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Features

  • Parallel isolation of small and large RNA
  • RNA purification fractionated by size:
  • Isolation of small RNA only (< 200 b),
  • Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
  • Isolation of total RNA (small and large RNA in one fraction)
  • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
  • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
  • NucleoSpin™ Filters for efficient sample homogenization
  • rDNase for efficient on-column removal of genomic DNA

Applications

  • Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
  • Purification of siRNA and large dsRNA from DICER reactions
  • Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Specifications

Format Mini Spin Column
For Use With (Application) Purification of siRNA and large dsRNA from DICER reactions
Includes NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
No. of Reactions 250 Reactions
RNase Yes
Sample Size Small RNA: < 200 nt, large RNA: > 200 nt
Test Time <45 min./6 Reaction and <35 min./6 Reaction
Yield 10μg small RNA, 95μg large RNA

Safety and Handling

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Hazard Category
  • Flammable liquid Category 2
  • Respiratory sensitiser Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 3
  • Carcinogenicity Category 2
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