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Proteintech™ Mouse IL-21 ELISA Kit

Product Code. 16841606 Shop All Proteintech Products
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Target Species:
Mouse
Unit Size:
96 tests
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Product Code. Target Species unitSize
16841606 Mouse 96 tests
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Product Code. 16841606 Supplier Proteintech™ Supplier No. KE1001296T
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Estimated Shipment: 17-09-2026
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KE10012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL21 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL21.

  • The assay recognizes mouse IL21. An antibody specific for mouse IL21 has been pre-coated onto the microwells.
  • The mouse IL21 protein in samples is captured by the coated antibody after incubation.
  • Following extensive washing, another antibody of biotinylated specific for mouse IL21 is added to detect the captured mouse IL21 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
  • Interleukin 21 (IL21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells.
  • IL21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL21 receptor (il21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL21.
  • IL21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells.
  • IL21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.

Specifications

Accession Number NP_068554
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity 0.5 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 60505
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID Q9ES17
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Product Identifier
  • 96TEST Mouse IL-21 ELISA Kit
Signal Word
  • Warning
Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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