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Invitrogen™ OxyBURST™ Green H2DCFDA, SE

Product Code. 11510286
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5 mg
Unit Size:
5mg
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Product Code. 11510286 Supplier Invitrogen™ Supplier No. D2935

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Amine-reactive assay reagent

OxyBURST™ Green H2DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) can be used to prepare oxidation-sensitive conjugates of wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, two acetates of OxyBURST™ Green H2DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield dihydrofluorescein conjugate. OxyBURST™ Green H2DCFDA conjugates are nonfluorescent until they are oxidized to corresponding fluorescein derivatives.

  • Ex/Em: approx. 492 - 495/517–527nm
  • Product is air sensitive (store under dry argon or nitrogen)
  • Product may be dissolved in DMSO, DMF, or ethanol for use
  • Indicator is cell permeant
  • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Refer to the Molecular Probes™ Handbook for additional product information.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 mg
Product Type ROS Indicator
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Product Identifier
  • 2',7'-DICHLORODIHYDROFLUO
Signal Word
  • Warning
Hazard Category
  • Acute toxicity Category 4
  • Reproductive toxicity Category 2
  • Skin sensitiser Category 1
Hazard Statement
  • H302-Harmful if swallowed.
  • H317-May cause an allergic skin reaction.
  • H361-Suspected of damaging fertility or the unborn child.
Precautionary Statement
  • P201-Obtain special instructions before use.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
Supplemental information
  • MIXTURE LIST-Contain: 2'',7''-DICHLORODIHYDROFLUO

For Research Use Only. Not for use in diagnostic procedures.

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