Invitrogen™ Calcium Orange™, AM, cell permeant - Special Packaging

• These indicators have uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues
• Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
• Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem
• Label (Ex/Em): Calcium Orange (549/576nm)
• Fluorescence intensity increase upon binding Ca²⁺ : approximately 3 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling