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IBA Lifesciences MHC I-Strep HLA-B*0702; CMV pp65 (TPRVTGGGAM)
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Product Code. 15623918 Shop All IBA Lifesciences Products
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Quantity:
40 μL
Unit Size:
40µL
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Product Code. Quantity unitSize
15623918 40 μL 40µL
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Product Code. 15623918 Supplier IBA Lifesciences Supplier No. 67027001

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Description

MHC class I molecule fused to a Twin-Strep-tag™ and refolded with a peptide

  • MHC I-Strep HLA-B*0702; CMV pp65 (TPRVTGGGAM) is a recombinantly expressed MHC class I molecule that presents a peptide from a human cytomegalovirus (CMV) phosphoprotein (pp65).
  • Our MHC I Streptamer™ approach is based on our Strep-tag technology and facilitates reversible antigen-specific T cell labeling.
  • A Twin-Strep-tag™ fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin™ backbone, resulting in the formation of MHC I Streptamers.
  • For multimerizing MHC I-Streps, Strep-Tactin™ TACS Agarose columns, Strep-Tactin™ Magnetic Microbeads or fluorophore-conjugated Strep-Tactin™ can be used.
  • This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements.
  • The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells.
  • The resulting label-free cells are highly viable and fully functional.

    • Specifications

    Capacity (Metric) 40 μL
    Kit Contents Fluorescent reagent for staining, magnetic T cell labeling and isolation
    Quantity 40 μL
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